Fig. 8.

Retigabine enhances the increase in intracellular [Ca²⁺] in cultured keratinocytes produced by carvacrol. a Representative Ca²⁺ signals, measured as increases in the 350:380-nm fluorescence signal in Fura-2 preloaded cells and recorded from two keratinocytes following two applications to each of 1 mM carvacrol for 3 min. The top trace shows representative responses to two successive applications of carvacrol. In the lower trace, the second carvacrol application was preceded by the addition of retigabine (Ret: bath concentration 10 μM). b The mean ratio of the second-to-first responses (R2/R1) following addition of Krebs’ solution (Carv + Krebs) or retigabine (Carv + Ret) before the second response. Bars are SEM, number of cells in brackets. The asterisk (**) indicated that the R2/R1 ratio is significantly greater when R2 was preceded by retigabine than when it was not (P < 0.01)