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. Author manuscript; available in PMC: 2014 May 30.
Published in final edited form as: Cell Rep. 2013 Apr 25;3(5):1503–1511. doi: 10.1016/j.celrep.2013.03.036

Figure 5. RA Controls Limb Tbx5 Independently of Heart Isl1.

Figure 5

(A–C) E8.5 mouse embryos analyzed by in situ hybridization (A and C) or stained with X-gal to detect Isl1-nlacZ expression (B).

(A) Isl1 mRNA in Rdh10 mutant (Rdh10 mut) versus wild-type (WT) embryos (ventral view). Arrows represent the posterior boundary of the heart field.

(B) Isl1-nlacZ expression in Raldh2−/−;Isl1-nlacZ+/− versus Isl1-nlacZ+/− embryos. Arrows represent the posterior boundary of the heart field (h).

(C) Assessment of heart expansion (h) and forelimb field Tbx5 expression (f) in wild-type, Isl1-nlacZ−/−, Raldh2−/−, and Raldh2−/−;Isl1-nlacZ−/− embryos.

See also Figures S4 and S5.

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