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Absorbance spectra (normalized to equal an IRDye 800CW concentration of 150 nM) of unattached IRDye 800CW and 800CW-tilmanocept conjugates with different IRDye 800CW densities: (black) IRDye 800CW-NHS-ester, (cyan, triangle) 0.7 dyes per tilmanocept, (red, circle) 1.5 dyes per tilmanocept, (green, hexagon) 2.3 dyes per tilmanocept, (blue, diamond) 2.9 dyes per tilmanocept, (pink, square) 3.8 dyes per tilmanocept.
