TABLE 1.
Primer Sequences Used for Reverse Transcriptase–polymerase Chain Reaction Screening for Drug Efflux and Uptake Transporters
| Transporter | Forward primer | Reverse primer | Control tissue |
|---|---|---|---|
| Efflux | |||
| MDR1 (ABCB1) | TCAGAATGGCAGAGTCAAGGAGC | TAGCAAGGCAGTCAGTTACAGTCCA | Liver |
| MRP2 (ABCC2) | AAGATTATAGAGTGCGGCAGCCCT | CCTGGGTAGTAGGTTCATGGGTGTT | Liver |
| MRP3 (ABCC3) | TCAGCACCCCTGCAGATCATCC | CACACTCTGGGGGTCAAGTTCCTC | Liver |
| MRP4 (ABCC4) | TCAAGTCCGTTCCGAAGGCA | GCATTTAACTGGTGGCCTGCA | Liver |
| MRP5 (ABCC5) | CCTGGAACCTCCGCTCAGAGAA | CTGGGTGCTGGTGTTTGGAAGTAG | Liver |
| MRP7 (ABCC7) | TCCAGCAGACCA TCTGCAAACG | GAGGTAGAGCCCCAGAGGAGAATGT | Liver |
| BCRP (ABCG2) | TGCATCTTGGCTGTCATGGC | TGTGCAACAGTGTGATGGCAAG | Liver |
| Uptake | |||
| OATP-B (SLC21A9) | AATAATGACCTGCTCCGAAACCG | ACTCAGAGGAGGTACTGCTGTGGCT | Liver |
| OCTN1 (SLC22A4) | TTCTTATCCATTGGTCTGGTCATGC | TGGGAACAACGAATTTCTCCACA | Kidney |
| OCTN2 (SLC22A5) | ATGCGGGACTACGACGAGGTGA | TGAAGGAGCCCAACAGCACACC | Liver |
| OAT3 (SLC22A8) | CCGCAACCTTCCCTGGTCGT | CCAGGTTCCACTCGGTCACAATG | Kidney |
| OCT3 (SLC22A3) | TTCCGCTTCCTGCAAGGTGTA | CCCCCTATAATTCCCAGGCG | Kidney |
| PGT (SLCO2A1) | CGCTCGGTCTTCGGCAACAT | AGGTGTACTGGTAGGGCTCGGAGAG | Kidney |
| PepT2 (SLC15A2) | AGCCAACAAAATGTCCATTGCG | GAAAGGTGGAGGAGATCAGCTCTGA | Kidney |