Table 1.
Effect of Plasminogen and Plasmin on Motility Parameters of Bull Spermatozoa.a
| Group | Motility (%) | Prog. Motility (%) | VCL (µm/s) | VSL (µm/s) | VAP (µm/s) | LIN (%) | STR (%) | WOB (%) | ALH (µm/s) | BCF (Hz) |
|---|---|---|---|---|---|---|---|---|---|---|
| Control | 41.4 ± 3.3 | 36.9 ± 3.5 | 129.8 ± 7.9 | 61.7 ± 4.8 | 79.1 ± 4.6 | 47.3 ± 2.5 | 73.8 ± 1.0 | 63.6 ± 2.5 | 2.7 ± 0.1 | 21.1 ± 1.3 |
| Plasminogen | 37.7 ± 2.2 | 32.8 ± 1.9 | 122.3 ± 6.0 | 52.1 ± 3.7 | 75.3 ± 3.8 | 46.4 ± 0.4 | 69.1 ± 5.2 | 65.6 ± 2.2 | 2.7 ± 0.1 | 19.0 ± 1.0 |
| Plasmin | 36.6 ± 1.8 | 32.4 ± 1.6 | 121.8 ± 5.6 | 60.1 ± 3.0 | 75.7 ± 3.3 | 50.1 ± 3.3 | 76.2 ± 2.3 | 64.4 ± 2.7 | 2.6 ± 0.1 | 18.0 ± 0.9 |
| P value | .36 | .34 | .64 | .18 | .77 | .27 | .08 | .73 | .67 | .71 |
Abbreviations: Prog. Motility: progressive motility; VCL: curvilinear velocity; VSL: straight-line velocity; VAP: average path velocity; LIN: linearity of the curvilinear trajectory; STR: straightness; WOB: wobble of the curvilinear trajectory (VAP/VCL); ALH: amplitude of lateral head displacement; BCF: beat cross-frequency.
a Motility parameters measured by computer-assisted sperm analysis (CASA). Bull frozen-thawed spermatozoa incubated in tyrode albumin lactate pyruvate (TALP) medium supplemented with plasminogen or plasmin (150 μg/mL). Data are mean ± standard error of the mean, and were analyzed by analysis of variance.