Fig. 1.

Necrotic and early defence responses induced by cellulose binding elicitor lectin (CBEL) in BAK1 and NADPH oxidase mutants. (A) bak1-4 and rbohD/F leaves were infiltrated with a CBEL solution at a concentration of 5 μg ml^–1; necrotic symptoms were observed 5 days later. (B) Kinetics of ROS production monitored over 40min on leaf discs of 4-week-old bak1-4, rbohD/F, and Col-0 plants; data are means of three biological replicates and bars represent standard deviation. (C) Total photon counts from the results shown on (B); bars with different letters indicate significantly different results (P < 0.05). (D) Detection of MAP kinase phosphorylation; total protein extracts from leaves infiltrated with CBEL (5 μg ml^–1) were analysed by Western blot using a p44/42 antibody and the membranes were stained by Ponceau red to control the equal loading of protein amount.