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. 2013 Jul 23;64(12):3735–3746. doi: 10.1093/jxb/ert206

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© The Author [2013]. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Fig. 4. — Histological observation in wheat cv. 92R137 at 72 hpi (A) and 120 hpi (B) after Pst CYR32 inoculation in the BSMV:γ- (a, a1), mock- (b, b1), and BSMV:TaRLP1.1as- (c, c1) pre-infected leaves. The same infection site was observed by white fluorescence microscopy for fungal growth (a, b, c) and epifluorescence microscopy for hypersensitive cell death (a1, b1, c1). (A) The spreading of secondary hyphae was suppressed (a, b), and mesophyll cells at the infection sites showed strong phenolic autofluorogen accumulation (a1, b1). However, secondary hyphae were formed (c) and autofluorescence was not obvious (c1) in the BSMV:TaRLP1.1as-infected leaves. Bars=20 μm. (B) Secondary hyphae were longer in the BSMV:TaRLP1.1as-infected leaves (c) than in the BSMV:γ- (a) and mock- (b) infected leaves. However, the area of autofluorescence was smaller in the BSMV:TaRLP1.1as-infected leaves (c1) than in the BSMV:γ- (a1) and mock- (b1) infected leaves. Bars=100 μm. SV, substomatal vesicle; IH, infection hypha; HMC, haustorial mother cell; SH, secondary hyphae; PAA, phenolic autofluorogen accumulation.