Fig. 2.

Relative expression of ripening-related genes and transcription factor-encoding genes in FaSHP-silenced (S) and -over-expressing (OE) fruits. ctrl and ctrl INTRON values are respectively from fruits either wild-type or agro-injected with the control construct pBINplus_intron. Fruits agro-injected at the G2(2) stage were left attached to the plant until attainment of pink colour. Each value represents the mean of five independent fruits. FaPG1, Polygalacturonase; FaPL, pectate lyase; FaEG1, endo-β-1,4-glucanase; FaCHS, chalcone synthase; FaPAL6, phenylalanine ammonia lyase, FaQR, quinone oxidoreductase; FaGalUR, d-galacturonate reductase. Expression data (means of the normalized expression) were obtained by real-time PCR analyses. Bars are the standard deviations from the means. Asterisks indicate values significantly different by Student’s t test from the control agro-injection (P < 0.05).