Fig. 5.

Transient silencing of ZmMAP65-1a reduces BR-induced H₂O₂ production. (A) H₂O₂ fluorescence in protoplasts transiently silencing ZmMAP65-1a. Protoplasts transfected with dsRNA against ZmMAP65-1a (RNAi) or distilled water as a control were treated with 10nM BR (+BR) or incubation medium (–BR) for 10min and then loaded with H2DCF-DA for 10min. H₂O₂ was visualized by confocal microscopy. Experiments were repeated at least three times with similar results. (B) Quantitation of the fluorescence intensity in (A). The protoplasts were treated as follows: 1, distilled water; 2, RNAi; 3, distilled water+BR; 4, RNAi+BR. Values are means ±SE of three different experiments. Means denoted by the same letter did not differ significantly at P <0.05 according to Duncan’s multiple range test.