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Ethyl methanesulfonate mutagenesis of Aspergillus oryzae. Aspergillus oryzae spore viability was reduced in a dose-dependent manner by ethyl methanesulfonate (EMS) treatment. Spore treatment with 1600 μg mL−1 EMS was used to generate the A. oryzae mutants screened for secretion of antibacterial compounds.
