Figure 1.

Effects of Psoralen on neurosphere formation of neural stem cells in vitro. Single NSCs at a density of 500 cells/well were cultured in normal growth medium (NGM) containing DMEM/F12 supplemented with hrFGF for 7 days to form various size of neurospheres (a). The neurospheres expressed NSC marker nestin (b). Single NSCs were exposed to NGM, DMSO (0.1%), and 10, 50, 100 nM Psoralen dissolved in DMSO (0.1%), respectively. Psoralen caused a significant decrease in frequency of neurosphere formation (c). Scale bars: 100 μm. Results were expressed as mean ± S.D. of six independent experiments. *P < 0.05 versus NGM.