Table 2.

Effect of extract, fractions, and compounds isolated from H.  pectinata against promastigotes of L. braziliensis.

Treatment	IC50 a (concentration ± S.E.M.)	Maximum effect (% ± S.E.M.)
Pentamidine	0.9 ± 0.03 µM/0.3 ± 0.01 µg/mL	93.5 ± 0.7**
EtOH extract	0.7 ± 0.1 µg/mL	91.6 ± 2.5**
MeOH : H2O fraction	3.9 ± 1.5 µg/mL	61.5 ± 1.2**
AcOEt fraction	0.4 ± 0.1 µg/mL	81.5 ± 5.9**
Hexane fraction	0.2 ± 0.1 µg/mL	90.0 ± 3.6**
1	6.9 ± 0.7 µM/2.5 ± 0.04 µg/mL	56.0 ± 0.8**
2	>100 µM/>36.0 µg/mL	48.8 ± 1.7**
3	>100 µM/>36.0 µg/mL	NA
4	5.4 ± 0.8 µM/2.0 ± 0.3 µg/mL	69.1 ± 2.7**
5	>100 µM/>20.8 µg/mL	NA
7	>100 µM/>31.4 µg/mL	NA

Data are reported as the mean ± S.E.M. Differences with a *P value < 0.05 were considered significant relative to the 0.1% DMSO group.

^aIC₅₀ is the concentration required to give 50% mortality, calculated by linear regression analysis from the Kc values at the concentrations employed.

NA: the compound is not active.