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. 2013 Jul 30;2013:686549. doi: 10.1155/2013/686549

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Copyright © 2013 Li Wang et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Cellular immune responses. Cellular immune responses were assessed with cells from spleens and lungs of immunized mice. Cells from spleens ((a), (c)) and lungs ((b), (d)) were stimulated with M2e peptides and M2e VLPs for 40 hours, and cytokine-secreting cell colonies were determined by ELISPOT assay. Each group has 3 mice. The asterisk (∗) indicates a significant difference between G2 and G1, G3 and G1, G4 and G1, and G5 and G1. The “+” indicates a significant difference between G3 and G2, G4 and G2, and G5 and G2.