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. 2012 Jul 2;33(12):1656–1664. doi: 10.1002/humu.22155

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© 2012 Wiley Periodicals, Inc.

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

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VPS33B and VIPAR interaction with VPS18. Confocal fluorescence photomicrographs of HEK293 cells cotransfected with (A) YFP-VPS33B and Myc-VPS18 found these proteins do not colocalize. However, (B) mCherry–VIPAR and Myc-VPS18 do colocalize in clusters in the absence of YFP-VPS33B. If all three proteins were cotransfected, then YFP-VPS33B and mCherry-VIPAR formed clusters and Myc-VPS18 assumed independent cytoplasmic distribution (C). Myc-VPS18 was immunostained with mouse monoclonal antibody anti-myc (Sigma) and anti-mouse ALEXA-568 conjugate secondary antibody (Invitrogen), both at a concentration of 1:400. Scale bar, 10 μm.