Figure 2.

KCNQ channel openers reduce contractility of bladder strips. (A) Example of the reduction in contraction amplitude by the KCNQ opener, flupirtine (20 μM). (B) Meclofenamic acid (MFA; 1 μM) also reduced contraction amplitude. (C, D, E) Summary bar charts for the effects of flupirtine and MFA on contractility measured as area under curve (AUC), frequency and contraction amplitude respectively. All experiments were carried out in the presence of tetrodotoxin (1 μM). *P < 0.05, significantly different from control. (F) Trace from a time-dependent control showing maintenance of spontaneous activity over several hours.