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. Author manuscript; available in PMC: 2013 Aug 19.
Published in final edited form as: Cell Metab. 2013 Mar 5;17(3):448–455. doi: 10.1016/j.cmet.2013.02.001

Figure 4. Decreased Anti-Inflammatory Activity of SIRT1-L107P.

Figure 4

(A) MIN6 cells were stably transduced with empty, wild-type (WT), or mutant SIRT1 L107P virus and stimulated with IL-1β or a combination of IL-1β and IFN-γ. iNOS mRNA expression relative to empty virus-transduced cells MIN6 cells, and NO formation following cytokine stimulation expressed as fold of unstimulated cells. Findings were verified with five separately produced sets of sirt1 WT and L107P-overexpressing cell lines each time at least in triplicate (mean ± SD, *p < 0.05; **p < 0.01).

(B) Wild-type and SIRT1-deficient mice were injected with five daily injections of streptozotocin (40 mg/kg). Glycemia and onset of diabetes (serum glucose >10 mM) were monitored for 16 days. Islet histology was visualized by HE and immunostaining for insulin (brown). The proportion of surface area that stained positive for insulin is shown. All data are presented as mean ± SEM. *p < 0.05; n = 6 for +/+ and +/−, and n = 5 for −/−.