Figure 5.

A subregion of the Sox2 serine-rich domain interacts with Nanog. (A) Schematic representation of the (FLAG)₃Sox2 constructs used for mapping the minimal Sox2 region involved in the interaction with Nanog. (B) E14/T cells were transfected with (HA)₃Nanog and the indicated (FLAG)₃Sox2 mutants. Immunoprecipitations were performed with an HA antibody as indicated and immunoblots probed with an anti-FLAG or anti-HA antibodies. I is 1% of input.

Source data for this figure is available on the online supplementary information page.

Source data for Figure 5 ^{(294.2KB, pdf)}