Skip to main content
. 2013 Jul 23;32(16):2287–2299. doi: 10.1038/emboj.2013.166

Figure 3.

Figure 3

Effect of MAP1B deficiency on LTD expression. fEPSPs were recorded from CA3-to-CA1 synapses and normalized to the average baseline value before LTD induction. (A) Time course of NMDAR-dependent LTD (1 Hz, 15 min) from MAP1B+/+ and MAP1B+/− slices. (B) Average responses collected from the last 5 min of the recording and normalized to the baseline. Left columns (LTD) correspond to the induced pathway. Only wild-type slices were significantly depressed with respect to their baseline (P=0.03, Wilcoxon test). Right columns (Control) correspond to the pathway that was not stimulated during the induction. (C) mGluR LTD was induced in MAP1B+/+ and MAP1B+/− slices with 50 μM of (RS)-3,5-DHPG for 5 min, as indicated. (D) Average responses collected from the last 5 min of the recording and normalized to the baseline. MAP1B+/+ slices were significantly depressed with respect to their baseline (P=0.03, Wilcoxon test). For all panels, ‘n’ represents the number of slices and ‘P’ the statistical significance value comparing the extent of depression in wild-type (black squares) versus MAP1B+/− (grey circles) slices (Mann–Whitney test). (E) Immunofluorescence images of MAP1B expression levels (green) and mCherry (red, lentiviral infection) from hippocampal primary neuronal cultures expressing the MAP1B shRNA (right panels) or the scrambled control (left panels). (F) Western blot showing the expression levels of MAP1B in organotypic slices from wild-type and heterozygous (MAP1B+/−), and from wild-type animals after the expression of the shRNA against MAP1B. (G) Time course of NMDAR-dependent LTD (500 pulses at 1 Hz) on wild-type organotypic hippocampal slice cultures from control (uninfected) neurons, neurons expressing a scrambled shRNA or from neurons expressing the shRNA against MAP1B, as indicated. (H) Average responses collected from the last 5 min of the recording and normalized to the baseline. Left columns (LTD) correspond to the induced pathway. Right columns (Control) correspond to the pathway that was not stimulated during the induction.