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. 2013 Aug 19;3:2462. doi: 10.1038/srep02462

Figure 1. The schematic of the 3D radial symmetry algorithm.

Figure 1

(a) The image generated by sampling the point spread function (PSF) of wide-field microscopy on a 3D grid with a lattice size of 20 nm. (b) The 3D CCD image simulated from the PSF image (a) with a signal-to-noise (S/N) ratio of 20. The blue crosses indicate the true center of the 3D particle. (c) The implementation procedure: scaling the 3D image in axial direction, and calculating the center position (the blue point) of the 3D single particle with the minimal distance to all gradient lines (the purple arrow lines).