. 2013 Aug;13(8):703–714. doi: 10.1089/ast.2013.0981

Table 3.

Gene Expression Profiles of IFN-γ, Perforin, Granzyme-B, NKG2D, NKG2A, NKp30, and NKp44 in NK Cells after 48 h Exposure to SMG

Gene	ΔΔCt (compared with RC)	ΔΔCt (compared with 1GC)
IFN-γ	3.53±0.75	1.96±0.33
	2^−ΔΔCt=2^−3.53=0.1(fold)^*	2^−ΔΔCt=2^−1.96=0.3(fold)^#
Perforin	2.09±0.97	2.52±0.43
	2^−ΔΔCt=2^−2.09=0.2(fold)^*	2^−ΔΔCt=2^−2.52=0.2(fold)^#
Granzyme-B	1.09±0.6	0.96±0.51
	2^−ΔΔCt=2^−1.09=0.5(fold)	2^−ΔΔCt=2^−0.96=0.5(fold)
NKG2A	2.34±0.3	2.05±0.76
	2^−ΔΔCt=2^−2.34=0.2(fold)^*	2^−ΔΔCt=2^−2.05=0.2(fold)^#
NKG2D	2.9±0.78	2.36±0.27
	2^−ΔΔCt=2^−2.9=0.1(fold)^*	2^−ΔΔCt=2^−2.36=0.2(fold)^#
NKp30	(−)0.28±0.45	0.23±0.61
	2^−ΔΔCt=2^0.28=1.2(fold)	2^−ΔΔCt=2^−0.23=0.9(fold)
NKp44	0.51±0.21	0.99±0.94
	2^−ΔΔCt=2^−0.51=0.7(fold)	2^−ΔΔCt=2^−0.99=0.5(fold)

Real-time quantitative (RT)-PCR using the SYBR Green random mixing method was employed to test the mRNA levels. GAPDH served as internal standard control. The data represent the mean±SD of six independent experiments. One-way ANOVA and LSD test, ^*p<0.05 compared with the RC group, ^#p<0.05 compared with the 1GC group.