Skip to main content
. 2013 Jul 26;24(8):766–775. doi: 10.1089/hum.2012.132

FIG. 3.

FIG. 3.

Ad5PTD(ASH1-SCG3-E1A) shows selective replication in neuroblastoma cell lines. (A) Neuroblastoma cell lines and a nonneuroblastoma cell line were transduced with Ad5PTD(ASH1-SCG3-E1), Ad5PTD(WT), or Ad5(MOCK) at 100 EVG/cell. Supernatants with progeny virus were collected after 4 days and added to 911 cells for titration of infectious progeny virus. (B) Three cell lines: SK-N-FI (neuroblastoma), HeLa (nonneuroblastoma tumor), and 1064Sk (normal fibroblasts) were transduced with Ad5PTD(ASH1-SCG3-E1A), Ad5PTD(WT), or Ad5(MOCK) at 10 EVG/cell; supernatants with progeny virus were harvested at various time points and added to 911 cells for titration of infectious progeny virus. (A and B) 911 cells exposed to progeny virus were harvested after 8 hr, followed by viral DNA isolation. Viral copies were determined by quantitative real-time PCR analysis. All data are shown as means+SD from three independent experiments, each with triplicate samples, (**p<0.01, ***p<0.001).

HHS Vulnerability Disclosure