FIG. 4.

Ad5PTD(ASH1-SCG3-E1A) kills drug-insensitive neuroblastoma cells expressing neural stem cell markers. A cisplatin-insensitive clone of SK-N-FI and doxorubicin-insensitive, etoposide-insensitive, and vincristine-insensitive clones of SH-SY-5Y were established through repeated cycles of culture with increasing drug concentration. (A–D) Drug-sensitive (black lines) and drug-insensitive (gray lines) neuroblastoma cells were treated with virus or drugs. Relative cell viability was analyzed after 4 days by MTS assay. (A) Oncolytic virus and cisplatin killing of SK-N-FI cells. (B) Oncolytic virus and etoposide killing of SH-SY-5Y cells. (C) Oncolytic virus and doxorubicin killing of SH-SY-5Y cells. (D) Oncolytic virus and vincristine killing of SH-SY-5Y cells. All data are shown as means±SD from four independent experiments, each with triplicate samples.