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. 2013 Aug;16(4):295–303. doi: 10.1089/rej.2013.1420

FIG. 1.

FIG. 1.

Reactive oxygen species (ROS) levels in the trophocytes and fat cells of young and old queens. (A) Red fluorescence indicates the presence of ROS in young and old queens. Purple fluorescence indicates nuclei. Arrows point to trophocytes. Arrowheads point to fat cells. Scale bar, 50 μm. (B) Quantification of ROS in young and old queens. Bars represent mean±standard error of the mean (SEM) (n=4). (C) Green fluorescence indicates the presence of ROS in young and old queens. Blue fluorescence indicates nuclei. Arrows point to trophocytes. Arrowheads point to fat cells. Scale bar, 50 μm. (D) Quantification of ROS in young and old queens. Bars represent mean±SEM (n=4). (E) The ROS levels in the supernatants of the trophocytes and fat cells of young and old queens. Bars represent mean±SEM (n=6). Asterisks indicate statistical significance as determined by two-sample t test (*) p<0.05).