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. 2012 Dec 12;21(9):957–964. doi: 10.1038/ejhg.2012.274

Figure 3.

Figure 3

Sub-cellular localization of KHDC3L and colocalization with NLRP7. (a–d) Simultaneous revelation of GFP-tagged wild-type KHDC3L (GFP-wtKHDC3L) construct with GFP signal (green) and KHDC3L C-terminal (C-term) antibody (Sigma, prestige) (red) in transfected HEK293T cells showing that the antibody recognizes the exogeneous protein found close to the nucleus. DNA was counterstained with DAPI (blue). (e, f) Absence of perinuclear staining in HEK293T cells transfected with the empty vector carrying GFP. (g–i) KHDC3L C-term antibody detected a juxta and perinuclear signal that colocalizes with NLRP7 (green) in EBV-transformed cells from a control subject. (j–l) A similar signal that colocalizes with NLRP7 was also detected by another N-terminal (N-term) KHDC3L antibody (Abgent). (m–x) Presence of KHDC3L signals with the N-term antibody and absence of signals with the KHDC3L C-term antibody in EBV-transformed cells from patients 481 and 654 with protein truncations at positions 100 and 108 amino acids, respectively. The truncated proteins in both patients colocalize with NLRP7 (m–o and s–u).