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. 2013 Jul 23;24(8):751–760. doi: 10.1089/hum.2013.051

FIG. 2.

FIG. 2.

Cytotoxicity of human T cells, in different E/T ratios mediated by different concentrations of diabody in a nonradioactive cytotoxicity assay. (A) Cytotoxicity of T cells in the presence of Nalm-6 mediated by the diabody. (B) Cytotoxicity of T cells in the presence of Ara-C-stimulating Nalm-6 mediated by the diabody. The diabody was at different concentrations (10, 1.0, and 0.1 pM). E/T ratio ranged from 25:1 to 3:1. (C, D) Lysis of target cells by T cells mediated by PBS, diabody, anti-CD3scFv, or anti-CD19scFv alone; a mixture of anti-CD3scFv and anti-CD19scFv; and anti-CD3×anti-Pgp diabody. The concentration of a variety of antibodies was 10 pM. (C) Target cells were Nalm-6. (D) Target cells were Nalm-6 stimulated by Ara-C. (E) Lysis of B-ALL cells (sample 1) or B-ALL cells (sample 1) stimulated by Ara-C in the presence of diabody (10 pM) or not. E/T ratio ranged from 25:1 to 3:1. Data shown are the mean±SD of experiments performed in quadruplicate. E/T ratio, effector-to-target cell ratio; PBS, phosphate-buffered saline; SD, standard deviation.