FIG. 5.

The functional significance of the involvement of ICAM-3. ICAM-3 expressed on Nalm-6 cells did not change after Nalm-6 cells were stimulated by Ara-C. ICAM-3 increased when Nalm-6 cells were stimulated by Ara-C co-cultured with T cells. When diabody was added, ICAM-3 increased significantly compared with the control (Nalm-6 group is a control) (A). The inhibition of the LFA-1–ICAM-3 interaction by anti-ICAM-3 mAbs and anti-LFA-1 mAbs reduced T-cell activation nearly to 45% on average. An isotype-matched control Ab did not decrease T-cell activation (B). *p<0.05 and **p<0.01 were compared with controls. mAbs, monoclonal antibodies.