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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Tissue Cell. 2013 May 3;45(4):253–260. doi: 10.1016/j.tice.2013.03.001

Figure 5. Ascorbate treatment enhances elastin and collagen deposition by pulmonary fibroblast within 3D Gelfoam constructs.

Figure 5

NNRLFs were cultured Gelfoam sponges in the presence (B and D) and absence (A and C) of ascorbate (10 mM) for 21 days. Cell-Gelfoam constructs were fixed, sectioned and stained with DAPI to visualize cell nuclei (blue) and with antibodies to elastin (A and B; red; 1:100 dilution) or type I collagen (C and D; green; 1:100 dilution). Images were collected using a 20x objective. Fluorescence exposure levels were kept constant for samples treated with and without ascorbate.