Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2014 Jul 1.

Published in final edited form as: Exp Physiol. 2013 Apr 12;98(7):1164–1177. doi: 10.1113/expphysiol.2013.072470

Example of whole-cell currents in response to voltage protocol as in Fig. 6A recorded in the same myocyte at different times during hypo-osmotic stress and its resolution. (Top) The timing of the application of Tyrode (Tyr) and hypo-osmotic 0.6 Na solutions (Normal and Low osmolarity). Ionic currents were recorded within ~1–2 min before application of 0.6 Na solution (▲), ~1–2 min before ( ) and ~3–6 min (△) after its removal. Current traces were normalized to each other at the end of depolarizing voltage step (I_{K, end}; ○) in order to highlight the relative changes in I_{K1, tail} currents. Fast capacitative transient upon repolarization is not shown. Insert: Quantification of the data shows that I_{K1, tail} currents are not affected by the hypo-osmotic stress itself but are significantly reduced (by ~5 fold; p<0.001) shortly after resolution of the shock.

Inline graphic — Example of whole-cell currents in response to voltage protocol as in Fig. 6A recorded in the same myocyte at different times during hypo-osmotic stress and its resolution. (Top) The timing of the application of Tyrode (Tyr) and hypo-osmotic 0.6 Na solutions (Normal and Low osmolarity). Ionic currents were recorded within ~1–2 min before application of 0.6 Na solution (▲), ~1–2 min before ( ) and ~3–6 min (△) after its removal. Current traces were normalized to each other at the end of depolarizing voltage step (I_{K, end}; ○) in order to highlight the relative changes in I_{K1, tail} currents. Fast capacitative transient upon repolarization is not shown. Insert: Quantification of the data shows that I_{K1, tail} currents are not affected by the hypo-osmotic stress itself but are significantly reduced (by ~5 fold; p<0.001) shortly after resolution of the shock.