Figure 3.

Effect of compound D on reduction of MMPs activities in culture media analyzed by gelatin zymography (a) and casein zymography (b). SW982 cells were treated with 1 ng/ml IL-1β in with or without the various concentrations of compound D or dexamethasone (Dexa). After 24 hours, cultured media were analyzed for the mixed activities of MMP-2 activity by gelatin zymography and MMP-1, -3, -13 by casein zymography, respectively. The activities were quantified by band densitometry as showed in the representative MMPs zymograms. All values are fold change compared to the control (C). The fold change of the mixed MMP-1, -3 and -13 activities are calculated from the total activities of pro- and active forms. Std = standard MMP-2