Table 5.
Compound | Fortified conc. (µg/mL) |
Intra-day | Inter-day | ||||
Observed conc. (µg/mL) |
Precision (%) |
Accuracy (%) |
Observed conc. (µg/mL) |
Precision (%) |
Accuracy (%) |
||
2.00 | 2.01 | 1.13 | 100.44 | 2.02 | 1.10 | 100.82 | |
5-HMF (1) | 5.00 | 5.25 | 0.38 | 104.93 | 5.27 | 0.98 | 105.35 |
10.00 | 9.88 | 0.11 | 98.75 | 9.86 | 0.23 | 98.63 | |
2.00 | 2.04 | 2.77 | 102.11 | 2.00 | 2.03 | 99.94 | |
Albiflorin (2) | 5.00 | 5.15 | 1.78 | 102.96 | 5.17 | 2.22 | 103.32 |
10.00 | 9.92 | 0.46 | 99.18 | 9.92 | 0.51 | 99.17 | |
10.00 | 10.10 | 1.04 | 100.97 | 10.14 | 1.22 | 101.42 | |
Paeoniflorin (3) | 25.00 | 25.20 | 0.74 | 100.79 | 25.17 | 1.77 | 100.66 |
50.00 | 49.88 | 0.15 | 99.76 | 49.89 | 0.44 | 99.78 | |
2.00 | 1.97 | 1.54 | 98.75 | 2.01 | 2.04 | 100.56 | |
Liquiritin (4) | 5.00 | 5.23 | 0.93 | 104.69 | 5.18 | 0.82 | 103.64 |
10.00 | 9.89 | 0.20 | 98.88 | 9.91 | 0.14 | 99.07 | |
4.00 | 4.19 | 2.02 | 104.81 | 4.06 | 1.87 | 101.58 | |
Nodakenin (5) | 10.00 | 10.41 | 2.85 | 104.13 | 9.75 | 2.83 | 97.46 |
20.00 | 19.90 | 0.81 | 99.49 | 20.11 | 0.64 | 100.57 | |
6.00 | 6.40 | 1.07 | 106.66 | 6.45 | 0.71 | 107.43 | |
Hesperidin (6) | 15.00 | 14.60 | 0.28 | 97.32 | 14.52 | 0.30 | 96.77 |
30.00 | 30.12 | 0.06 | 100.40 | 30.15 | 0.06 | 100.51 | |
4.00 | 4.18 | 1.38 | 104.53 | 4.17 | 1.04 | 104.37 | |
Glycyrrhizin (7) | 10.00 | 10.07 | 1.32 | 100.73 | 10.05 | 0.40 | 100.46 |
20.00 | 19.93 | 0.28 | 99.64 | 19.94 | 0.07 | 99.71 |