Skip to main content
. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2012 Jul 31;69(9):625–643. doi: 10.1002/cm.21051

Figure 4. Cortactin-KD cells contain many LE/Lys hybrid organelles and have little perturbation in Golgi structure or volume.

Figure 4

Transmission electron microscopy was performed on 50 nm thick cellular sections. A, B. Representative images of the overall Golgi morphology in SCC61-scrambled (A) and SCC61 cortactin-KD1 (B) cells. Note the increase in LE/Lys and lamellar bodies (LB) within KD cells. NUC = nucleus. Zooms of the Golgi shown below A and B. An increase in trans-Golgi-associated vesicles in KD cells is indicated with a bracket. C, D. Representative images of the cytoplasmic contents of control (C) and cortactin-knockdown (D) SCC61 cells. LE/Lys and LB are indicated by * in C and D). Scale bar = 500 nm. E. % Cytoplasmic volume occupied by Golgi or LE/Lys was analyzed by point counting stereology of random thin sections and plotted. N = 3 sections, from 2 independent blocks. For representative HeLa images, see Fig S2B–E. Mean+/−SE shown. * p<0.05, **p<0.01.