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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2012 Jul 31;69(9):625–643. doi: 10.1002/cm.21051

Figure 5. Depletion of cortactin leads to accumulation of Mannose-6-Phosphate Receptor (M6PR) in a late endosomal compartment and depletion from the Golgi.

Figure 5

A and B. HeLa cells were stained for M6PR (CI-M6PR) and either Golgi marker Giantin (A) or late endosomal marker Rab7 (B). Boxed areas are zoomed to show colocalization of M6PR with Giantin or Rab7. Colocalized single pixels (white) were visualized using colocalization ImageJ plugin. Scale bars = 20 µm. C. Images were analyzed for % colocalization of M6PR with Giantin or Rab7. n=3; >15 cells per independent experiments. Data are presented as box and whiskers plots with the box indicating the 25th and 75th percentiles, solid line indicating the median, and the whiskers indicating the 95% confidence intervals.***p<0.001