Skip to main content
. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2012 Jul 31;69(9):625–643. doi: 10.1002/cm.21051

Figure 6. Inhibition of late endosomal/lysosomal trafficking leads to a compact Golgi morphology.

Figure 6

A. Representative immunoblot of Rab7 expression levels (top panels) in SCC61 parental cells and those transiently transfected with non-targeting control siRNA (NTC) or Rab7a-specific siRNA, along with a β-actin control. B. Rab7-KD reduces Golgi size in SCC61 cells. Representative images (green=GM130, blue=Hoechst) and analysis. C. Chloroquine treatment in SCC61 cells reduces Golgi size. Representative images and analysis of SCC61 parental cells treated with either vehicle (water, “control”) or chloroquine (60 µM, “CQ”) for 12 hours. D. Rab7-KD causes an increase in Golgi size in HeLa cells. Representative images and corresponding analysis. Scale bar= 25 µm. n=3, with >30 cells per independent experiment. Data are presented as box and whiskers plots with the box indicating the 25th and 75th percentiles, solid line indicating the median, and the whiskers indicating the 95% confidence intervals.*p<0.05.