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. Author manuscript; available in PMC: 2013 Aug 19.
Published in final edited form as: J Neuroimmune Pharmacol. 2011 Dec 10;7(2):388–400. doi: 10.1007/s11481-011-9325-0

Figure 7.

Figure 7

Feline macrophage conditioned medium (fMCM) from FIV-inoculated macrophages (106 TCID50/ml) induced a destabilization of intracellular calcium that was inhibited in the presence of LM11A-31. Conditioned medium from feline choroid plexus macrophages collected 3 days after inoculation with FIV was applied to primary feline neurons at a dilution of 1:5 in aCSF. Fluorescence intensity (calcium concentration) is pseudocolored to highlight changes (white>red>yellow>green>blue>dark blue). A. The conditioned medium caused a modest acute rise in intracellular calcium (0.5 min) which failed to recover leading to the appearance of focal calcium increases and swelling in dendrites by 5.8 min followed by a large calcium destabilization and dendritic beading after 66 min. B. Cultures pre-treated with 10 nM LM11A-31 for 10 min and challenged with the same feline macrophage-conditioned medium as above showed a similar acute rise but minimal delayed destabilization.