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. Author manuscript; available in PMC: 2013 Aug 19.
Published in final edited form as: Science. 2013 Jan 25;339(6118):460–464. doi: 10.1126/science.1227299

Fig. 4. Distinct responses of WT, NLS, and NES phosphosite mutants of Msn2 to natural stresses.

Fig. 4

Single-cell responses of WT, NLS 4A, NLS 4E, and NES 2A to glucose limitation (A), osmotic stress (B), and oxidative stress (C) (n: ~50 cells, each stress condition). Representative single-cell time traces of Msn2 nuclear translocation are shown. Asterisks emphasize the conditions under which the mutants fail to distinguish two different stresses. Quantification of the time traces is presented in Fig. S4. (D) Time traces of WT Msn2-mCherry and mutant Msn2-YFP, monitored in the same cells, in response to glucose limitation (black – WT, blue – NLS 4A, green – NLS 4E, red – NES 2A). More single-cell traces are shown in Fig. S5.