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. Author manuscript; available in PMC: 2013 Aug 19.
Published in final edited form as: Traffic. 2008 Jun 28;9(9):1458–1470. doi: 10.1111/j.1600-0854.2008.00782.x

Figure 1. Mutagenesis of the viral kinases.

Figure 1

(A) Illustration of UL13 and US3 mutations made in the PRV genome (PRV-GS847: strain Becker previously modified to express mRFP1 fused to the VP26 capsid protein). (B) Western blot analysis of extracellular purified virions. Viral kinases were detected with anti-UL13 or anti-US3 antibodies. A revertant of the double-kinase-null virus (PRV-GS1555) in which both kinase deletion alleles were repaired (rev) expressed both kinases similar to the wild type (wt). Representative proteins of each component of the virus were also examined for structural incorporation: VP5 (capsid), UL37 (inner tegument), VP22 (outer tegument), gD (envelope). (C) Kinase mutants propagate with reduced kinetics. PK15 cells were infected at MOI=5, and the cells and supernatants were harvested at the indicated times post infection. Viral titers were quantitated by plaque assay.