Figure 4.
Blast internalization of AbBNPs as a function of AbBNP size (· = 50 nm; ο = 100 nm) and antibody surface density ratio (Ab/BNP = 0–200) for CRLF2+ (A,B) and control (C,D) blasts ( n = 7). The optimal AbBNP sizes and Ab/BNP ratios for internalization by CRLF2+ blasts was 50 nm with 10 Ab/BNP (A) and 100 nm with 25 Ab/BNP (B), and this was reached in less than 1 h (Supporting Information Figure 2). The optimal AbBNP sizes and Ab/BNP ratios for internalization by control blasts were similar (C,D). Representative flow cytometry plots are shown for optimal AbBNP sizes and Ab/BNP ratios.
