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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Insect Biochem Mol Biol. 2013 Jun 6;43(8):755–767. doi: 10.1016/j.ibmb.2013.05.008

Figure 3. Substrate induced current inAeNAT5 expressing oocytes.

Figure 3

A. Representative current trace recorded during perfusion of selected amino acids and amino acid derivatives. All substrates were added at 1 mM final concentration during the periods indicated by the black bars above the actual trace. 98 mM NaCl was used as the major salt. A voltage clamp mode with a holding transmembrane voltage of −50 mV was used during this recording. The single letter code corresponds to the canonical abbreviations for amino acids. Other abbreviations are: DOPA, L-3,4-dihydroxyphenylalanine; 5HTP, 5-Hydroxytryptophan; 5-HT. 5-Hydroxytryptamine; DA, dopamine; dM, D-Methionine; GABA, γ-Amino butyric acid; Tau, Taurine; OA, Octopamine; TA, Tyramine. B. Result of statistical evaluation of AeNAT5 induced currents (bars are the normalized average value for n > 3, different batches/oocytes). The neurotransmitters are indicated by gray font. C. Amino acid induced responses in control DW injected oocytes at the same amplitude and time scale as in panel A and insert.