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. Author manuscript; available in PMC: 2014 Jul 15.
Published in final edited form as: J Immunol. 2013 Jun 7;191(2):773–784. doi: 10.4049/jimmunol.1300113

Figure. 4. Macrophage phenotypes and distribution in necrotic and suppurative granulomas from macaques with active TB.

Figure. 4

Individual panels show serial 5-μm thick sections of representative necrotic and suppurative granulomas. Hematoxylin and eosin staining (top) of a necrotic (A) and suppurative (F) granulomas with pseudocolored representation (bottom) indicating lymphocyte-rich (cyan), epithelioid macrophage-rich (purple) and necrotic (yellow, panel A) or suppurative (yellow, panel F) regions. Black boxes indicate the regions depicted at higher magnification in images C-E and G-J. B, G. Macrophage-specific stains including HAM56 (red), CD163 (green) and calprotectin-stained neutrophils (blue). White box indicates the region depicted at higher magnification in images C-E. C, H. iNOS (green) and Arg1 (red) expression with nuclei (blue). D, I. CD68 (red) and eNOS (green) expression with nuclei (blue). E, J. Arg2 (green) expression with nuclei (blue). Scale bars represent 100 μm.