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. Author manuscript; available in PMC: 2014 Jul 15.
Published in final edited form as: J Immunol. 2013 Jun 7;191(2):773–784. doi: 10.4049/jimmunol.1300113

Figure. 6. Macrophage phenotypes and distribution in fibrocalcific granulomas from macaques with latent TB.

Figure. 6

Individual panels show serial 5-μm thick sections of a representative necrotic granuloma. A. Hematoxylin and eosin (top) with pseudocolored representation (bottom) indicating the outer fibrotic region (cyan), fibro-calcific interface (purple) and central region containing mineralized material (yellow) that shattered during cutting. Dashed line indicates portion of the fibrocalcific interface that is separated from the rest of the granuloma and is present as an artifact in A. Black box indicates the region depicted at higher magnification in images C-E. B. Macrophage-specific stains including HAM56 (red), CD163 (green) and calprotectin-stained neutrophils (blue). Grey line outlines the tissue edge denoted by the dashed line in A where the fibrocalcific interface has separated from the surrounding tissue and reflected over the other side. White box indicates the region depicted at higher magnification in images C-E. C. iNOS (green) and Arg1 (red) expression with nuclei (blue). D. CD68 (red) and eNOS (green) expression with nuclei (blue). E. Arg2 (green) expression with nuclei (blue). Scale bars represent 100 μm.