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. 2001 Jul 3;98(15):8507–8512. doi: 10.1073/pnas.151251898

Figure 2.

Figure 2

Imino proton spectrum (11.1–14.1 ppm) (A) and expanded NOESY (150-ms mixing time) contour plots (B–D) of the [PhIP]dG⋅dC 11-mer duplex adduct in H2O buffer at 1°C. The right panels are plotted at 10 times lower level relative to the left panels. The assignments for the imino resonances in the major conformer are given in A and D. Resonances belonging to the minor conformer are designated by Xs in A. (B) NOE connectivities between the 1 [dG18(NH1)] and 2 [dG16(NH1)] imino protons and PhIP(N-CH3). (C) PhIP-DNA cross peaks are 3, dG18(NH1)-PhIP(H5); 4, dG16(NH1)-PhIP(H5); and 5, dG16(NH1)-PhIP(H7). DNA–DNA cross peaks are lettered as follows: A and A′, dG18(NH1)-dC5(NH2-4e,b), where e and b designate exposed and hydrogen-bonded, respectively; B, dG18(NH1)-dA19(H2); C, dG16(NH1)-dA15(NH2-6); D and D′, dG16(NH1)-dC7(NH2-4e,b); E, dG16(NH1)-dA15(H2); and F, [PhIP]dG6(NH1)-dC7(NH2-4e). (D) Imino–imino connectivities are traced by the solid line from dG13 to dG16 and by the dashed line from dG18 to dG21. The boxes indicate the loss of connectivities between the [PhIP]dG6 imino and the dG16 and dG18 iminos.