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Relative growth of E. coli thiF− strains (VJS2895) at 37°C in M9/glucose/AMP/KAN medium (500 ml) containing 0.4% glucose, 100 mg AMP, 50 mg KAN, 0.1 mM IPTG, and 0.0084% arginine. ThiF Mutant + Thz (▴): VJS2895 was transformed with thiF(C184S)SGH in pET22b(+) + additive (50 μM thiazole); wild type (Wt) (□): VJS2895 was transformed with thiFSGH in pET22b(+); pET22b(+) (⧫): VJS2895 was transformed with pET22b(+); ThiF mutant (○): VJS2895 was transformed with thiF(C184S)SGH in pET22b(+).
