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. Author manuscript; available in PMC: 2013 Aug 19.
Published in final edited form as: J Recept Signal Transduct Res. 2009;29(0):182–194. doi: 10.1080/10799890902976933

Figure 2.

Figure 2

Evaluation of the specificity of the DMR signal induced by EGF in UPCI-37B cells. (A) Effect of the tyrosine kinase inhibitor AG 1478 on the EGF-induced DMR signal. The cells were preincubated with increasing concentrations of AG 1478 before introducing EGF (100 ng/mL). The real-time DMR response was continuously monitored for 45 min. (B) Dose-response curve of the AG 1478 effect on EGF-induced DMR response. The inhibitory effects of AG 1478 on the P-DMR signal induced by EGF were normalized to the vehicle control wells in the presence of EGF but without compound. The IC50 was calculated from the dose-response curve using Prism 4.0 software. (C) The effect of SU 1498 (10 μM), a VEGFR inhibitor as a negative control, on the DMR response induced by EGF (100 ng/mL).