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. Author manuscript; available in PMC: 2013 Aug 19.
Published in final edited form as: J Recept Signal Transduct Res. 2009;29(0):182–194. doi: 10.1080/10799890902976933

Figure 6.

Figure 6

Evaluation of cell-based optical signature assays for high-throughput screening (HTS). (A) To evaluate the suitability and optimal conditions of the biosensor EGFR signaling assay for HTS, the Z′ factors and signal-to-background ratio (S/B) were calculated and plotted against increasing concentrations of EGF in UPCI-37B SCCHN cells. (B) Z′ factors were calculated for EGF-induced N-DMR and P-DMR signal in A549 cells and plotted along with N-DMR signal against EGF concentrations. The Z′ factor incorporates both the signal window and the well-to-well variations and is a criterion for evaluating the suitability and conditions of the assay for HTS. A Z′ factor between 0.5 and 1 indicates that the assay is robust for screening chemical modulators of the targets.