Table 1. X-ray data and refinement statistics.
| gp15 C-terminal truncation mutant (residues 1–261) | ||||
|---|---|---|---|---|
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|
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| Full length gp15 | Triclinic, Pt derivative | |||
|
|
|
|||
| Trigonal, native | Trigonal, native | Peak | Inflection point | |
| X-ray wavelength (Å) | 1.0 | 0.97941 | 1.07195 | 1.07228 |
| Space group | P32 | P1 | P1 | |
| Cell dimensions | ||||
| a, b, c (Å) | 100.68, 100.68, 155.891 | 65.87, 76.31, 93.89 | 65.89, 77.01, 94.16 | |
| α, β, γ (°) | 90, 90, 120 | 109.04, 104.62, 89.30 | 109.01, 104.43, 88.94 | |
| Resolutiona (Å) | 2.7 (2.75–2.7) | 3.2 (3.31–3.2) | 3.6 (3.73–3.6) | 3.6 (3.73–3.6) |
| Rmergea | 0.078 (0.27) | 0.077 (0.21) | 0.076 (0.17) | 0.073 (0.27) |
| I/σa | 20.0 (3.3) | 18.7 (5.9) | 15.9 (5.6) | 17.3 (3.6) |
| Completenessa (%) | 93 (85) | 92 (72) | 79 (58) | 77(51) |
| Redundancya | 5.5 (2.3) | 3.8 (3.5) | 2.3 (2.2) | 2.3 (2.1) |
|
| ||||
| Refinement statistics | ||||
|
| ||||
| Crystal form | Trigonal, native | Triclinic, native | ||
| Resolution (Å) | 2.7 | 3.2 | ||
| No. of reflections | 45,176 | 25,229 | ||
| No. of atoms | ||||
| Protein | 10,452 | 10,145 | ||
| Water | 72 | 0 | ||
| Rwork/Rfree | 0.207/0.252 | 0.171/0.202 | ||
| Root-mean-square deviations | ||||
| Bond length (Å) | 0.010 | 0.009 | ||
| Bond angles (°) | 1.30 | 1.22 | ||
| Average B-factor (Å2) | 64 | 62 | ||
| Ramachandran plot (%) | ||||
| Favored | 90 | 90 | ||
| Outliers | 1.9 | 2.0 | ||
a
Numbers in parentheses represent values in the highest-resolution shell.