Table 1.

Yields of a–c crosslinking in aN214C/cM65C-membranes and the effects of DCCD treatment

DCCD treatment*	Efficiency of a–c crosslinking†
None	>97%
10 μM, 2 h‡	96.5% (±0.4%)
100 μM, 0.5 h	81.4% (±0.7%)

^*Similar experiments with [¹⁴C]DCCD showed labeling of 1.8 c/F_OF₁ for 10 μM [¹⁴C]DCCD and 9 c/F_OF₁ for 100 μM [¹⁴C]DCCD (Table 2). 

^† Crosslinking efficiency (±SD) was estimated from immunoblot detection of subunit a (see Fig. 2). Controls using varied amounts of nonoxidized sample established that the assay provided a linear response to subunit a for the amounts remaining in oxidized samples. 

^‡ Exposure to MgATP before crosslinking had no significant effect on the yield of a–c product.