Fig. 8.

Immunohistochemical NHE3 staining reveals normal localization of NHE3 in jejunal and colonic apical membrane of NHERF1^−/−mice. The NHE3 localization at the apical border of the jejunum (a) and colon (b) was qualitatively similar in WT (left panels) and NHERF1^−/− (right panels) mice with considerable variability in the signal intensity within each group of mice. Images are representative examples from five independent experiments using tissue from three different mouse couples. Inserts show a ×10 magnification of a part from the image shown in the larger panels