| 5 × TRAP buffer | 5.0 µl |
| dNTP (50 mM) | 1.0 µl |
| TS primer | 1.0 µl |
| Taq-DNA polymerase | 0.5 µl |
| Protein lysate (1–5 mg) | |
| H2O | to 25.0 µl |
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