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. 2013 Jul 29;110(33):13546–13551. doi: 10.1073/pnas.1301463110

Fig. 2.

Fig. 2.

SIRT2 is a replication stress response protein. (A and B) hTERT RPE-1 cells were transfected with NT, ATR, ATRIP, or SIRT2 siRNA, treated with 3 mM HU for 24 h (arrested), and released into nocodazole for 10 h (released). DNA content was analyzed by using flow cytometry. (B) The percentage (mean and SEM) of cells that completed DNA synthesis in three replicate experiments is shown. **P < 0.01. (C and D) U2OS cells were transfected with NT, CHK1, or SIRT2 siRNA and processed 72 h later for RPA70 staining by indirect immunofluorescence microscopy. (D) The percentage (mean and SEM) of RPA70 foci positive cells from four replicate experiments is shown. *P < 0.05 and **P < 0.01. (E) Soluble and chromatin fraction of U2OS cells transfected with NT or SIRT2 siRNA for 72 h. Chromatin-bound RPA70:ORC2 ratio of representative blot from three independent experiments is shown. See also Fig. S2.