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CDK9 complements the replication stress response impairment of SIRT2 deficiency. U2OS cells stably expressing an empty vector, FLAG-HA-CDK9 WT or K48Q were transfected with NT, ATR, ATRIP, or SIRT2 siRNA, split 1:4 48 h later, and treated 24 h later with or without 3 mM HU for 24 h before assaying for cell viability using WST-1 reagent. Mean and SEM from four replicas is shown. *P < 0.05 and **P < 0.01.
